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Image Search Results
Journal: PLoS ONE
Article Title: The cellular prion protein interacts with and promotes the activity of Na,K-ATPases
doi: 10.1371/journal.pone.0258682
Figure Lengend Snippet: Exposure to 20 nM ouabain caused a reduction in the steady-state levels of PrP C , ATP1A1 and ATP1A2—but not ATP1A3—in a manner that cannot be reversed by concomitant incubation with YM24476. In contrast, co-incubation of cells with ouabain and A6185 rescued the reduction in ATP1A1 levels and caused the appearance of signals detected with the PrP C - and ATP1A2-directed antibodies that migrated with different apparent molecular weights than the dominant signals for these proteins observed in untreated cells. Finally, the intensity of relatively fast migrating signals detected with a GFAP-directed antibody increase under conditions that favor intracellular calcium-dependent calpain activity.
Article Snippet: To quantify ATP1A1 and PRNP transcript levels in ReN VM cells following vehicle or Ouabain treatment at 10nM, 30nM and 50nM conditions, cells were treated every other day over a period of 7 days in biological triplicates and qPCR was conducted in technical duplicates, each reaction containing 50 ng of cDNA, TaqMan master mix (catalog number 4427788,
Techniques: Incubation, Activity Assay