sarcomeric alpha actinin (saa) (thermo fisher, cat Search Results


95
Developmental Studies Hybridoma Bank rabbit α hpat1
Rabbit α Hpat1, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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99
Thermo Fisher gene exp actb mm02619580 g1
Gene Exp Actb Mm02619580 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
Thermo Fisher horseradish peroxidase substrate pierce ecl2
Horseradish Peroxidase Substrate Pierce Ecl2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher goat anti rabbit igg antibody conjugated to horseradish peroxidase invitrogen cat
Goat Anti Rabbit Igg Antibody Conjugated To Horseradish Peroxidase Invitrogen Cat, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher gene exp hif1a hs00153153 m1
Gene Exp Hif1a Hs00153153 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Santa Cruz Biotechnology anti α tubulin mouse monoclonal
Anti α Tubulin Mouse Monoclonal, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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92
Thermo Fisher gene exp atp1a3 hs00958036 m1
Exposure to 20 nM ouabain caused a reduction in the steady-state levels of PrP C , ATP1A1 and ATP1A2—but not <t>ATP1A3—in</t> a manner that cannot be reversed by concomitant incubation with YM24476. In contrast, co-incubation of cells with ouabain and A6185 rescued the reduction in ATP1A1 levels and caused the appearance of signals detected with the PrP C - and ATP1A2-directed antibodies that migrated with different apparent molecular weights than the dominant signals for these proteins observed in untreated cells. Finally, the intensity of relatively fast migrating signals detected with a GFAP-directed antibody increase under conditions that favor intracellular calcium-dependent calpain activity.
Gene Exp Atp1a3 Hs00958036 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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99
Thermo Fisher gibco glucose solution
Exposure to 20 nM ouabain caused a reduction in the steady-state levels of PrP C , ATP1A1 and ATP1A2—but not <t>ATP1A3—in</t> a manner that cannot be reversed by concomitant incubation with YM24476. In contrast, co-incubation of cells with ouabain and A6185 rescued the reduction in ATP1A1 levels and caused the appearance of signals detected with the PrP C - and ATP1A2-directed antibodies that migrated with different apparent molecular weights than the dominant signals for these proteins observed in untreated cells. Finally, the intensity of relatively fast migrating signals detected with a GFAP-directed antibody increase under conditions that favor intracellular calcium-dependent calpain activity.
Gibco Glucose Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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98
Thermo Fisher mouse anti vinculin
Exposure to 20 nM ouabain caused a reduction in the steady-state levels of PrP C , ATP1A1 and ATP1A2—but not <t>ATP1A3—in</t> a manner that cannot be reversed by concomitant incubation with YM24476. In contrast, co-incubation of cells with ouabain and A6185 rescued the reduction in ATP1A1 levels and caused the appearance of signals detected with the PrP C - and ATP1A2-directed antibodies that migrated with different apparent molecular weights than the dominant signals for these proteins observed in untreated cells. Finally, the intensity of relatively fast migrating signals detected with a GFAP-directed antibody increase under conditions that favor intracellular calcium-dependent calpain activity.
Mouse Anti Vinculin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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97
Thermo Fisher cell culture grade bovine erum albumin
Exposure to 20 nM ouabain caused a reduction in the steady-state levels of PrP C , ATP1A1 and ATP1A2—but not <t>ATP1A3—in</t> a manner that cannot be reversed by concomitant incubation with YM24476. In contrast, co-incubation of cells with ouabain and A6185 rescued the reduction in ATP1A1 levels and caused the appearance of signals detected with the PrP C - and ATP1A2-directed antibodies that migrated with different apparent molecular weights than the dominant signals for these proteins observed in untreated cells. Finally, the intensity of relatively fast migrating signals detected with a GFAP-directed antibody increase under conditions that favor intracellular calcium-dependent calpain activity.
Cell Culture Grade Bovine Erum Albumin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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97
Thermo Fisher gene exp ppargc1a mm00447183 m1
Exposure to 20 nM ouabain caused a reduction in the steady-state levels of PrP C , ATP1A1 and ATP1A2—but not <t>ATP1A3—in</t> a manner that cannot be reversed by concomitant incubation with YM24476. In contrast, co-incubation of cells with ouabain and A6185 rescued the reduction in ATP1A1 levels and caused the appearance of signals detected with the PrP C - and ATP1A2-directed antibodies that migrated with different apparent molecular weights than the dominant signals for these proteins observed in untreated cells. Finally, the intensity of relatively fast migrating signals detected with a GFAP-directed antibody increase under conditions that favor intracellular calcium-dependent calpain activity.
Gene Exp Ppargc1a Mm00447183 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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99
Thermo Fisher alexa488 conjugated collagen
Exposure to 20 nM ouabain caused a reduction in the steady-state levels of PrP C , ATP1A1 and ATP1A2—but not <t>ATP1A3—in</t> a manner that cannot be reversed by concomitant incubation with YM24476. In contrast, co-incubation of cells with ouabain and A6185 rescued the reduction in ATP1A1 levels and caused the appearance of signals detected with the PrP C - and ATP1A2-directed antibodies that migrated with different apparent molecular weights than the dominant signals for these proteins observed in untreated cells. Finally, the intensity of relatively fast migrating signals detected with a GFAP-directed antibody increase under conditions that favor intracellular calcium-dependent calpain activity.
Alexa488 Conjugated Collagen, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Exposure to 20 nM ouabain caused a reduction in the steady-state levels of PrP C , ATP1A1 and ATP1A2—but not ATP1A3—in a manner that cannot be reversed by concomitant incubation with YM24476. In contrast, co-incubation of cells with ouabain and A6185 rescued the reduction in ATP1A1 levels and caused the appearance of signals detected with the PrP C - and ATP1A2-directed antibodies that migrated with different apparent molecular weights than the dominant signals for these proteins observed in untreated cells. Finally, the intensity of relatively fast migrating signals detected with a GFAP-directed antibody increase under conditions that favor intracellular calcium-dependent calpain activity.

Journal: PLoS ONE

Article Title: The cellular prion protein interacts with and promotes the activity of Na,K-ATPases

doi: 10.1371/journal.pone.0258682

Figure Lengend Snippet: Exposure to 20 nM ouabain caused a reduction in the steady-state levels of PrP C , ATP1A1 and ATP1A2—but not ATP1A3—in a manner that cannot be reversed by concomitant incubation with YM24476. In contrast, co-incubation of cells with ouabain and A6185 rescued the reduction in ATP1A1 levels and caused the appearance of signals detected with the PrP C - and ATP1A2-directed antibodies that migrated with different apparent molecular weights than the dominant signals for these proteins observed in untreated cells. Finally, the intensity of relatively fast migrating signals detected with a GFAP-directed antibody increase under conditions that favor intracellular calcium-dependent calpain activity.

Article Snippet: To quantify ATP1A1 and PRNP transcript levels in ReN VM cells following vehicle or Ouabain treatment at 10nM, 30nM and 50nM conditions, cells were treated every other day over a period of 7 days in biological triplicates and qPCR was conducted in technical duplicates, each reaction containing 50 ng of cDNA, TaqMan master mix (catalog number 4427788, Thermo Fisher) and one of the following TaqMan assay kits (Thermo Fisher) targeting of human PRNP (catalog number Hs01920617_s1), ATP1A1 (catalog number Hs00933601_m1), ATP1A2 (catalog number Hs01560076_g1) or ATP1A3 (catalog number Hs00958036_m1).

Techniques: Incubation, Activity Assay